Figure 1. Western blot analysis of IGFBP5 using anti-IGFBP5 antibody (PB9711). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: U20S Whole Cell Lysate, Lane 2: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP5 antigen affinity purified polyclonal antibody (Catalog # PB9711) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP5 at approximately 23 kDa. The expected band size for IGFBP5 is at 31 kDa.
Figure 1. Western blot analysis of IGFBP5 using anti-IGFBP5 antibody (PB9711). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. Lane 1: U20S Whole Cell Lysate, Lane 2: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP5 antigen affinity purified polyclonal antibody (Catalog # PB9711) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP5 at approximately 23 kDa. The expected band size for IGFBP5 is at 31 kDa.
Anti-IGFBP5 Antibody Picoband(r)
PB9711
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Canine, Human, Monkey, Rabbit
TargetIGFBP5
Overview
- SupplierBoster Bio
- Product NameAnti-IGFBP5 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID3488
- Target nameIGFBP5
- Target descriptioninsulin like growth factor binding protein 5
- Target synonymsIBP5; IBP-5; IGF-binding protein 5; IGFBP-5; insulin-like growth factor-binding protein 5
- HostRabbit
- IsotypeIgG
- Protein IDP24593
- Protein NameInsulin-like growth factor-binding protein 5
- Scientific DescriptionBoster Bio Anti-IGFBP5 Antibody Picoband® catalog # PB9711. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Canine, Human, Monkey, Rabbit
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203