Western blot analysis of IKBKE expression in Raji cell lysate using anti-IKBKE antibody (M01816). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKE antigen affinity purified polyclonal antibody (Catalog # M01816) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IKBKE.
Western blot analysis of IKBKE expression in Raji cell lysate using anti-IKBKE antibody (M01816). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKE antigen affinity purified polyclonal antibody (Catalog # M01816) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IKBKE.
Anti-IKBKE Monoclonal Antibody
M01816
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIKBKE
Overview
- SupplierBoster Bio
- Product NameAnti-IKBKE Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAFAO-9
- FormulationLiquid
- Gene ID9641
- Target nameIKBKE
- Target descriptioninhibitor of nuclear factor kappa B kinase subunit epsilon
- Target synonymsI-kappa-B kinase epsilon; IKKE; IKK-E; IKK-epsilon; IKKI; IKK-i; IKK-related kinase epsilon; inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa-B kinase subunit epsilon
- HostRabbit
- IsotypeIgG
- Protein IDQ14164
- Protein NameInhibitor of nuclear factor kappa-B kinase subunit epsilon
- Scientific DescriptionBoster Bio Anti-IKBKE Monoclonal Antibody catalog # M01816. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203