Anti-IL-10 [9D7]

Absolute Antibody

Anti-IL-10 [9D7]

7

The six 9D7 Fab CDR loops interact with a discontinuous IL-10M1 epitope comprised of residues 71-83 and 125-137. 9D7 non-competitively antagonises IL-10R1 binding.

9D7 has been used in ELISA experiments in order to specifically detect IL-10 and show higher circulating levels of IL-10 in patients with acute EBV-induced mononucleosis (Taga 1995). Further, 9D7 has been used to immunoprecipitate IL-10 from the supernatants of melanoma cells in order to study the production of IL-10 by melanoma cells (Qiyuan Chen 1994). A fab fragment derived from 9D7 has been used to study the noncompetitive antibody neutralisation of IL-10 by x-ray crystallography (Josephson 2002).

ImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Neutralisation; ELISA; IHC; IF; IP; crystallisation

Research Use Only

Monoclonal

9D7

IL10

interleukin 10

CSIF; cytokine synthesis inhibitory factor; GVHDS; IL-10; IL10A; interleukin-10; T-cell growth inhibitory factor; TGIF

Rabbit

IgG

Interleukin-10

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine.

-20°C,2°C to 8°C

12352203