Anti-IL-12p40 [Antibody 80]

Absolute Antibody

Anti-IL-12p40 [Antibody 80]

9

This antibody specifically binds an epitope between amino acid residues 253 and 286 of the common subunit p40 of the human IL-12 and IL-23. IL-12Rbeta1 binding to IL-12 and IL-23 is mediated via the common p40 subunit. IL-12 is a cytokine secreted by acti

This antibody neutralized the binding of IL-12 to IL-12Rbeta2 and IL-23 to IL-23R but did not inhibit the binding of IL-12 or IL-23 or IL-12p40 or IL-12p80 to IL-12Rbeta1. The human IgG version of this antibody binds IL-12 and IL-23 with a binding affinity of Kd= 292 pM and Kd= 280 pM respectively. This antibody was reported to inhibit IL-12 induced IFN-gamma release by NK-92 cells and human PBMCs. This antibody can also inhibit IL-23 induced IL-17 secretion by murine splenocytes. The therapeutic efficacy of this antibody was studied in the skin inflammation mouse model. This antibody was reported to significantly decrease epidermal thickness and reduced erythema and induration caused by IL-23. This antibody is capable of detecting recombinant IL-23 and native IL-23 produced by SAC stimulated PBMCs in an ELISA. This antibody showed reduced binding to IL-23 mutants D265A and R266A in an ELISA (US8563697).

Functional Assay, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

Antibody 80

IL12B

interleukin 12B

CLMF; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; IL12, subunit p40; IL-12B; IMD28; IMD29; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF2

Rabbit

IgG

Interleukin-12 subunit beta

Human

-20°C,2°C to 8°C

12352203