Anti-IL-13 [M1295]

Absolute Antibody

Anti-IL-13 [M1295]

7

This antibody binds human interleukin-13 at the surface of helices A and D close to N- and C-termini of the IL-13 polypeptide chain. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosi

This antibody can bind human IL13 in an ELISA. The affinity of original IgG1 antibody for wild type human IL-13 was determined by BIAcore to be 5.7 +/- 1.3 pM (PMID: 20226193). Preclinical studies confirm the functional effects of M1295-mediated neutralization of human IL-13 in human cell based in vitro assays using mechanistically relevant endpoints associated with the pathogenesis of asthma, atopic dermatitis, and eosinophil-mediated disease (US8399630).

ELISA, Neutralisation/Blocking

ELISA; neutralize; therapeutic

Research Use Only

Monoclonal

M1295

IL13

interleukin 13

IL-13; interleukin-13; P600

Rabbit

IgG

Interleukin-13

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original mouse antibody m836 was generated by immunizing BALB/c mice with a natural variant of human IL-13 known as R130Q. The humanized version H2L6 was generated by grafting CDRs of mouse antibody onto selected human heavy chain and light chain frameworks and finally site-directed mutagenesis of two methionine in the CDRs of heavy chain with binding affinity optimization resulted in the generated of M1295.

-20°C,2°C to 8°C

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