Figure. Western blot analysis of IL-17 using anti-IL-17 antibody (RP1019). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Mouse IL-17 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-17 antigen affinity purified polyclonal antibody (Catalog # RP1019) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-17 at approximately 30KD. The expected band size for IL-17 is at 30KD.
Figure. Western blot analysis of IL-17 using anti-IL-17 antibody (RP1019). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Mouse IL-17 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-17 antigen affinity purified polyclonal antibody (Catalog # RP1019) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-17 at approximately 30KD. The expected band size for IL-17 is at 30KD.
Anti-IL17/IL17A Antibody Picoband(r)
RP1019
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetIl17a
Overview
- SupplierBoster Bio
- Product NameAnti-IL-17 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteOther applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- Applications SupplierELI, WB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID16171
- Target nameIl17a
- Target descriptioninterleukin 17A
- Target synonymsCtl; Ctla; Ctla8; Ctla-8; cytotoxic T-lymphocyte-associated antigen 8; Il; IL-; Il17; IL-17; IL-17A; interleukin-17A
- HostRabbit
- IsotypeIgG
- Protein IDQ62386
- Protein NameInterleukin-17A
- Scientific DescriptionBoster Bio Anti-IL17/IL17A Antibody catalog # RP1019. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse
- Reactivity SupplierMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Adipose-Derived Stem Cell Exosomes Inhibit Hypertrophic Scaring Formation by Regulating Th17/Treg Cell Balance. Wang H et al., 2022, Biomed Res IntRead more