Anti-IL-1A [18F4.2C8]

Absolute Antibody

Anti-IL-1A [18F4.2C8]

7

This antibody binds human Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1al

The original mouse IgG antibody binds human IL-1A with a binding affinity of Kd= 0.6 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 8.90E-11M. This antibody was used in combination with 1B12.4H4 (anti-IL-1beta) antibody for the generation of anti-human IL-1alpha/beta DVD-Ig molecule that had therapeutic potential (PMID: 20068402).

Functional Assay, ELISA

ELISA; functional assay

Research Use Only

Monoclonal

18F4.2C8

IL1A

interleukin 1 alpha

hematopoietin-1; IL1; IL-1 alpha; IL-1A; IL1-ALPHA; IL1F1; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha

Rabbit

IgG

Interleukin-1 alpha

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizing mice with recombinant human IL-1 alpha.

-20°C,2°C to 8°C

12352203