Anti-IL-1B [10G11]

Absolute Antibody

Anti-IL-1B [10G11]

7

This antibody binds mouse Interleukin-1 beta, which is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production,

The original mouse IgG antibody binds mouse IL-1B with a binding affinity of Kd= 0.23 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 3.70E-10M. This antibody was used in combination with 9H10 (anti- mouse IL-1A) antibody for the generation of anti-mouse IL-1alpha/beta DVD-Ig molecule (US7612181).

Functional Assay, ELISA

ELISA; functional assay

Research Use Only

Monoclonal

10G11

Il1b

interleukin 1 beta

IL-; IL-1 beta; Il-1b; IL-1beta; interleukin-1 beta

Rabbit

IgG

Interleukin-1 beta

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

The original antibody was generated by immunizing IL-1alfa/bèta double KO mice with mouse IL-1 beta.

-20°C,2°C to 8°C

12352203