Anti-IL-1B [1B12.4H4]

Absolute Antibody

Anti-IL-1B [1B12.4H4]

7

This antibody binds human Interleukin-1 beta, which is a potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production,

The original mouse IgG antibody binds human IL-1B with a binding affinity of Kd= 0.26 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 5.30E-10M. This antibody was used in combination with 18F4.2C8 (anti-IL-1alpha) antibody for the generation of anti-human IL-1alpha/beta DVD-Ig molecule that had therapeutic potential (PMID: 20068402).

Functional Assay, ELISA

ELISA; functional assay

Research Use Only

Monoclonal

1B12.4H4

IL1B

interleukin 1 beta

catabolin; IL-1; IL-1 beta; IL1beta; IL1-BETA; IL1F2; interleukin 1beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Rabbit

IgG

Interleukin-1 beta

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizing mice with recombinant human IL-1 beta.

-20°C,2°C to 8°C

12352203