Anti-IL-2 [LNKB-2]

Absolute Antibody

Anti-IL-2 [LNKB-2]

9

This antibody binds human IL-2 at the 59-72 site of the IL-2 sequence.

This antibody was quantified by ELISA and used for latex-agglutination analysis of human IL-2. It was measured to bind human IL-2 with high affinity (Ka ~ 3 x 108 M-1) (Lunev et al., 1990; PMID: 1718463). This antibody has been used successfully for WB and FACS (Stratieva-Taneeva et al., 1993; PMID: 8359822). The crystal structure characterization of the Fab version of this antibody was performed (Fokin et al., 2000; PMID: 11040993). Further crystallization of the Fab version of this antibody with an antigenic peptide revealed that the antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the alpha-helical character of the epitope fragment (Afonin et al., 2001; PMID: 11468348).

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, Other Application

Research Use Only

Monoclonal

LNKB-2

IL2

interleukin 2

aldesleukin; IL-2; interleukin-2; involved in regulation of T-cell clonal expansion; lymphokine; T cell growth factor; TCGF

Human

IgG4

Interleukin-2

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203