Anti-IL-2R Beta chain [Mik-beta 1]

Absolute Antibody

Anti-IL-2R Beta chain [Mik-beta 1]

7

This antibody recognizes and binds the beta chain of the IL-2 receptor and completely inhibits the IL-2 binding to the beta chain. The beta subunit is involved in receptor mediated endocytosis and transduction of the mitogenic signals of IL2.

Mik-beta 1 inhibited binding of iodine labelled IL-2 to YTS cells in a dose dependent manner. Mik-beta 1 also successfully immunoprecipitated beta chain of the IL-2R from radio iodinated YTS extract. Biotinylated Mik-beta 1 also efficiently competes with IL-2 for binding to solubilized beta chain. Flow cytometry was used to study the cellular disctribution of beta chain, as determined by mik-beta 1 staining. This antibody completely inhibited IL-2 dependent T cell growth in presence of anti-Tac. Two-color flow cytometric analysis using anti-beta chain mAbs clearly showed that approximately 10% of peripheral blood lymphocytes manifest the beta chain and that this population is mainly composed of Leu-19+ NK cells (PMID: 2467293). Mik beta 1 antibody acts synergistically with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells (PMID: 8335891). A recombinant single chain immunotoxin of Mik-beta 1 and Pseudomonas derived exotoxin PE40 was selectively cytotoxic for cells expressing high levels of IL-2R beta (p75) subunit (PMID: 1401913).

Flow Cytometry, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

Inhibit; Block; IP; ELISA; FC

Research Use Only

Monoclonal

Mik-beta 1

IL2RB

interleukin 2 receptor subunit beta

CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL15RB; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; IMD63; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-15 receptor subunit beta; interleukin-2 receptor subunit beta; P70-75; p75

Human

IgG1

Interleukin-2 receptor subunit beta

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizing BLAB/c mice with YTS cells, a human NK-like cell line that highly expresses the human IL-2R beta chain as immunogen.

-20°C,2°C to 8°C

12352203