Anti-IL-2R Beta chain [Mik-beta 1]

Absolute Antibody

Anti-IL-2R Beta chain [Mik-beta 1]

7

This antibody recognizes and binds the beta chain of the IL-2 receptor and completely inhibits the IL-2 binding to the beta chain. The beta subunit is involved in receptor mediated endocytosis and transduction of the mitogenic signals of IL2.

Mik-beta 1 inhibited binding of iodine labelled IL-2 to YTS cells in a dose dependent manner. Mik-beta 1 also successfully immunoprecipitated beta chain of the IL-2R from radio iodinated YTS extract. Biotinylated Mik-beta 1 also efficiently competes with IL-2 for binding to solubilized beta chain. Flow cytometry was used to study the cellular disctribution of beta chain, as determined by mik-beta 1 staining. This antibody completely inhibited IL-2 dependent T cell growth in presence of anti-Tac. Two-color flow cytometric analysis using anti-beta chain mAbs clearly showed that approximately 10% of peripheral blood lymphocytes manifest the beta chain and that this population is mainly composed of Leu-19+ NK cells (PMID: 2467293). Mik beta 1 antibody acts synergistically with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells (PMID: 8335891). A recombinant single chain immunotoxin of Mik-beta 1 and Pseudomonas derived exotoxin PE40 was selectively cytotoxic for cells expressing high levels of IL-2R beta (p75) subunit (PMID: 1401913).

Flow Cytometry, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

Inhibit; Block; IP; ELISA; FC

Research Use Only

Monoclonal

Mik-beta 1

IL2RB

interleukin 2 receptor subunit beta

CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL15RB; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; IMD63; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-15 receptor subunit beta; interleukin-2 receptor subunit beta; P70-75; p75

Mouse

IgG2a

Interleukin-2 receptor subunit beta

Human

Human

The original antibody was generated by immunizing BLAB/c mice with YTS cells, a human NK-like cell line that highly expresses the human IL-2R beta chain as immunogen.

-20°C,2°C to 8°C

12352203