Anti-IL-33 [874F7-Hu1]

Absolute Antibody

Anti-IL-33 [874F7-Hu1]

9

This antibody binds an epitope between amino acid residues 41-70 of the human IL-33. This antibody can also cross react with mouse and cynomolgus monkey IL-33. Interleukin 33 (IL-33) is a multifunctional cytokine which is constitutively expressed in struc

The binding characterization and determination of cross-reactivity of this antibody to human, mouse and cynomolgus monkey IL-33 was done using ELISA. This antibody binds human, mouse and cynomolgus monkey IL-33 with a binding affinity of Kd=1.13 nM, Kd= 0.332 nM and Kd=1.12 nM respectively. This antibody can block the binding of IL-33 to its receptor ST2 with an IC50 of 0.451 nM. This antibody can effectively inhibit IL-33 induced 16-6 expression in HUVEC cells, IL-5 and IL-13 secretion by KU812 cells. This antibody also showed inhibitory effect on IL-33 induced PBMC and NK cells secretion of IFN gamma. This antibody was reported to effectively inhibit mouse peripheral blood IL-15 secretion in vivo. This antibody could also inhibit the number of eosinophils stimulated by human IL-33 in the peripheral blood of mice. The in vivo pharmacodynamic activity of this antibody was studied using spleen weighing assay (WO2022063281).

Functional Assay, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

874F7-Hu1

IL33

interleukin 33

C9orf26; DVS27; DVS27-related protein; IL1F11; interleukin-1 family member 11; interleukin-33; NFEHEV; NF-HEV; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules

Rabbit

IgG

Interleukin-33

Human, Monkey, Mouse

-20°C,2°C to 8°C

12352203