Figure. Western blot analysis of IL-4 using anti-IL-4 antibody (RP1009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane: Recombinant Human IL-4 Protein 0.5ng, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-4 antigen affinity purified polyclonal antibody (Catalog # RP1009) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-4 at approximately 14KD. The expected band size for IL-4 is at 14KD.
Figure. Western blot analysis of IL-4 using anti-IL-4 antibody (RP1009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane: Recombinant Human IL-4 Protein 0.5ng, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-4 antigen affinity purified polyclonal antibody (Catalog # RP1009) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-4 at approximately 14KD. The expected band size for IL-4 is at 14KD.
Anti-Interleukin-4 IL4 Antibody Picoband(r)
RP1009
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL4
Overview
- SupplierBoster Bio
- Product NameAnti-IL-4 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteBy Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- Applications SupplierELI, IHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID3565
- Target nameIL4
- Target descriptioninterleukin 4
- Target synonymsB cell growth factor 1; B_cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4; interleukin 4 variant 2; interleukin-4; lymphocyte stimulatory factor 1; pitrakinra
- HostRabbit
- IsotypeIgG
- Protein IDP05112
- Protein NameInterleukin-4
- Scientific DescriptionBoster Bio Anti-Interleukin-4 IL4 Antibody catalog # RP1009. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Reactivity SupplierHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages. Li K et al., 2022 Jan 20, J NanobiotechnologyRead more