Anti-IL-4 [hu3B9], Human Fab fragment, His-Tagged, kappa

Absolute Antibody

Anti-IL-4 [hu3B9], Human Fab fragment, His-Tagged, kappa

9

This antibody is specific for human IL-4 and binds a non-contiguous epitope.

The binding affinity of the original format and Fab fragment of this antibody were evaluated by ELISA. The original format of this antibody (mouse IgG1) exhibited a <0.2 nM Kd (~0.18 nM). The Kd of its Fab fragment was <0.3 nM. This antibody successfully inhibited IL-4 binding to its receptor, as evaluated using 125 I-rhIL4 binding to the gibbon cell line, MLA. This antibody successfully inhibited lymphocyte proliferation - the original format of this antibody inhibited 3 H-thymidine incorporation by human peripheral blood T lymphocytes stimulated with 133 pM IL-4 and human tonsillar B lymphocytes stimulated by 167 pM IL-4. The IC50 for inhibition of T cell proliferation was 30 pM, and for B cell proliferation 103 pM. The corresponding values for the Fab fragment were 108 and 393 pM. This antibody successfully inhibited CD23 induction - the original format of this antibody inhibited CD23 expression on human tonsil B lymphocytes stimulated with 8.3 pM IL-4 with an IC50 value of 136 pM. This antibody successfully inhibited IgE secretion - the original format and the Fab fragment of this antibody inhibited IgE secretion in the presence of 1.7 nM IL-4, giving IC50 values of 1.9 and 5.0 nM, respectively. The experiment was repeated using a lower concentration of IL-4, 667 pM, which reduced the IC50 value to 0.65 nM for the original format of the antibody. The humanized version of this antibody effectively inhibited the binding of IL-4 receptor-Fc fusion protein to IL-4 in a dose-dependent manner - at lower concentrations (10-20 nM), it achieved 50% inhibition, while at higher concentrations (1 microM), it achieves greater than 90% inhibition. In a pharmacokinetics experiment performed on male Sprague Dawley rats, the humanized version of this antibody exhibited low inter-animal variability with a slow plasma clearance value of 0.5 mL/h/kg and an approximate 11 days half-life with a biphasic disappearance pattern (US5914110A).

ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

hu3B9

IL4

interleukin 4

B cell growth factor 1; B_cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4; interleukin 4 variant 2; interleukin-4; lymphocyte stimulatory factor 1; pitrakinra

Human

Interleukin-4

Human

-20°C,2°C to 8°C

12352203