Anti-IL-5 [JES1-39D10]

Absolute Antibody

Anti-IL-5 [JES1-39D10]

7

This antibody recognizes and binds human interleukin-5.

This antibody can effectively block IL-5 induced HL-60 histamine content and TF-1 proliferation. 39D10 was also evaluated for its ability to neutralize the ILJ-dependent growth of eosinophilbasophil colonies (i.e., both histamine-positive and histaminenegative Eo-type) in methylcellulose culture. This antibody did not cross-react with mouse IL-5 in indirect ELISA (PMID: 1706953). This antibody was used in immunohistochemical method to study the localisation and phenotype of individual cytokine producing cells in synovial biopsy specimens in rheumatoid arthritis (PMID:7677442). This antibody was also used in immunocytochemical and a flow cytofluorimetric method to detect intracellular IFN-g, IL-4 and IL-5 in T-cell clones, peripheral blood mononuclear cells PBMC and bronchoalveolar lavage fluid BALF cells (PMID: 9134033). The original rat IgG2a antibody binds human IL-5 with a binding affinity of Kd= 53 pM (PMID: 7613159). This antibody can be used in combination with JES1-5A10 for the detection of cytokines in a capture ELISA (PMID: 16827896). Humanized version of this antibody CMX5-2 and CMX5-5 had lower affinities for IL-5 than 39D10, with Kd values that were 1.4 times the Kd for 39D10 (PMID: 8844835).

Flow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking

Block; ELISA; IHC; FC; immunocytochemistry

Research Use Only

Monoclonal

JES1-39D10

IL5

interleukin 5

B-cell differentiation factor I; colony-stimulating factor, eosinophil; EDF; eosinophil differentiation factor; IL-5; interleukin-5; T-cell replacing factor; TRF

Human

IgG1

Interleukin-5

This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizing a male lewis rat with semi-purified recombinant mammalian-expressed IL-5.

-20°C,2°C to 8°C

12352203