Anti-IL-6 [1.26.4]

Absolute Antibody

Anti-IL-6 [1.26.4]

9

This antibody binds specifically to chicken IL-6, an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine (cytokine produced from muscle). Further, the antibody shows cross-reactivity with human and murine IL-6.

The specificity of this antibody (IgG2b) towards the native protein chIL-6 was confirmed in ELISA analysis (Scott et al, 2006; pmid: 16930722). The antibody detected the denatured chIL-6 by western blot. The antibody showed inhibitory activity on murine IL-6 on a B9 hybridoma cell bioassay. The bioassay was run with varying concentrations of the antibody in order to determine the effective concentration for neutralizing IL-6. Further, recombinant forms of chicken, human and murine IL-6 were all tested in the bioassay. The antibody was effective in neutralizing all three recombinant IL-6 proteins. (Scott et al, 2006; pmid: 16930722).

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

1.26.4

IL6

interleukin 6

CHIL-6; IL-6; interleukin 6 (interferon, beta 2); interleukin-6

Mouse

IgG2b

Interleukin-6

Chicken

-20°C,2°C to 8°C

12352203