Anti-IL-6 [61H7]

Absolute Antibody

Anti-IL-6 [61H7]

9

This antibody recognizes and binds interleukin-6 on the top of the four-helix bundle near the N and C termini. Here the VL domain sterically interferes with the C-terminal domain of IL-6R (residues 195-295). The overlap of the 61H7 epitope and the site I

This antibody is recommended for identification of IL-6 in the sample. The ability of this antibody to compete with biotinylated IL-6 for binding to captured IL-6R was investigated using competitive ELISA. The in-vitro potency of the antibody was determined using a cell-based neutralizing bioassay using the B9 and 7TD1 cell line, which require IL-6 for survival and proliferation and respond to very low concentration of IL-6 (PMID: 27129274).

ELISA

Research Use Only

Monoclonal

61H7

IL6

interleukin 6

B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HGF; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL-6; interferon beta-2; interleukin BSF-2; interleukin-6

Human

IgG1

Interleukin-6

This chimeric human antibody was made using the variable domain sequences of the original Fab format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203