Anti-IL-6 [68F2]

Absolute Antibody

Anti-IL-6 [68F2]

7

This antibody recognizes and binds interleukin-6 near the binding site I near the N-terminal part of alpha-helix 1 and the C-terminal part of alpha-helix 4 of the four-helix bundle, as well as the long loop between alpha-helices 1 and 2. This binding inte

This antibody is recommended for identification of IL-6 in the sample. The ability of this antibody to compete with biotinylated IL-6 for binding to captured IL-6R was investigated using competitive ELISA. The in-vitro potency of the antibody was determined using a cell-based neutralizing bioassay using the B9 and 7TD1 cell line, which require IL-6 for survival and proliferation and respond to very low concentration of IL-6 (PMID: 27129274).

ELISA

ELISA

Research Use Only

Monoclonal

68F2

IL6

interleukin 6

B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HGF; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL-6; interferon beta-2; interleukin BSF-2; interleukin-6

Rabbit

IgG

Interleukin-6

This chimeric rabbit antibody was made using the variable domain sequences of the original Fab format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was generated by immunizations of Llama glama with recombinant IL-6 followed by construction of phage display library, selection, screeing and purification.

-20°C,2°C to 8°C

12352203