
Figure 1. Western blot analysis of IL1F10 using anti-IL1F10 antibody (A09268-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat RH35 cell lysates, Lane 4: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1F10 antigen affinity purified polyclonal antibody (Catalog # A09268-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1F10 at approximately 17KD. The expected band size for IL1F10 is at 17KD.
Anti-IL1F10 Antibody Picoband(r)
A09268-2
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetIl1f10
Overview
- SupplierBoster Bio
- Product NameAnti-IL1F10 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID362077
- Target nameIl1f10
- Target descriptioninterleukin 1 family member 10
- Target synonymsinterleukin 1 family, member 7; interleukin-1 family member 10
- HostRabbit
- IsotypeIgG
- Protein IDD4A9I5
- Protein NameInterleukin-1
- Scientific DescriptionBoster Bio Anti-IL1F10 Antibody Picoband® catalog # A09268-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203