Anti-IL2R [AHT54]

Absolute Antibody

Anti-IL2R [AHT54]

7

AHT54 recognises a determinant close to the IL-2 binding site.

AHT54 has been used in ELISAs for the detection of soluble human IL-2R in response to different forms of helminthic and protozoal infections (O. Josimovic et al. 1988). AHT54 has also been shown to have anti-proliferative activities, and chimeric AHT54 antibodies with a human contant region have an increased inhibitory effect on proliferation of T lymphocytes (Rose 1992).

Flow Cytometry, ELISA, Neutralisation/Blocking

ELISA; FC; Block

Research Use Only

Monoclonal

AHT54

IL2RA

interleukin 2 receptor subunit alpha

CD25; IDDM10; IL-2 receptor subunit alpha; IL2R; IL-2R subunit alpha; IMD41; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

Rabbit

IgG

Interleukin-2 receptor subunit alpha

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned.

-20°C,2°C to 8°C

12352203