Anti-Influenza A nucleoprotein [aNP-VHH1]

Absolute Antibody

Anti-Influenza A nucleoprotein [aNP-VHH1]

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This antibody is specific for an epitope on the influenza virus A NP. The epitope is thought to overlap with a protein region responsible for several host protein interactions, including importin-a and Mx proteins. Immunoprecipitation confirmed no reactiv

The original version of this antibody (alpaca VHH) was confirmed to target NP antigens in an immunoprecipitation assay. The specificity of this antibodys binding has been validated using both a His tag and mCherry attachment. Furthermore, X-ray crystallography revealed its binding with IVA NP, where it binds to the NP body domain. Mini-genome assays have shown that this antibody not only interferes with Influenza A replication but also prevents the nuclear import of RNPs. Additionally, the antibody has been utilized for the immunoprecipitation of IVA NP and in Western blotting, further confirming its inhibitory effect on the nuclear localization of its target ribonuclear protein, disrupting the viruss function (Ashour et al., 2015; PMID: 25540369). Flow cytometry, in conjunction with negative-stain EM, demonstrated the interference of influenza A vRNP nuclear import by the VHH version of this antibody. This interference occurs through the masking of the nuclear localization sequence of NPs, proposing a model for the inhibition of vRNP nuclear import (Hanke et al., 2016; PMID: 27965447).

Electron Microscopy, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

aNP-VHH1

NP

nucleoprotein

FLUAVs5gp1; nucleoprotein

Human

IgG1

Nucleoprotein

This chimeric human antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203