Anti-Integrin alpha-4/beta-7 [Vedolizumab (LDP-02)]

Absolute Antibody

Anti-Integrin alpha-4/beta-7 [Vedolizumab (LDP-02)]

9

This antibody is specific for human alpha4beta7 integrin and does not cross react with alpha(4)beta(1) and alpha(E)beta(7) integrins. This antibody does not bind majority of memory CD4(+) T lymphocytes (60%), neutrophils, and most monocytes. It binds to a

The binding characterization of this antibody for human alpha 4 beta 7 integrin was done using flow cytometry on Hut-78 cells (US7147851B1). In a double-blind, placebo-controlled trial this antibody was more effective than placebo for the induction of clinical and endoscopic remission in patients with active ulcerative colitis (PMID: 15958805). In a phase II clinical trial, it was reported that this antibody is well tolerated in patients with active Crohns disease and therapy with this antibody had a dose-dependent beneficial effect on clinical remission (PMID: 18829392). This antibody exclusively binds alpha4 beta7 integrin and modulates inflammation in the gastrointestinal tract without inducing the systemic immunosuppression. This antibody binds memory CD4(+) T and B lymphocytes with sub nanomolar potency (EC(50) = 0.3-0.4 nM). This antibody inhibits adhesion of alpha(4)beta(7)-expressing cells to mucosal addressing cell adhesion molecule 1 (median inhibition concentration [IC(50)] = 0.02-0.06 microg/ml) and fibronectin (IC(50) = 0.02 microg/ml), but not vascular cell adhesion molecule 1. The pharmacologic properties of vedolizumab, in conjunction with the gastrointestinal tropism of alpha(4)beta(7) integrin function, make it a suitable for treatment of patients with inflammatory bowel diseases (PMID: 19509315).

Flow Cytometry

Research Use Only

Monoclonal

Vedolizumab (LDP-02)

ITGA4

integrin subunit alpha 4

269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49D; IA4; integrin alpha-4; integrin alpha-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha

Mouse

IgG2b

Integrin alpha-4

Human

-20°C,2°C to 8°C

12352203