Anti-Integrin alpha 5 beta 1 [SG/19]

Absolute Antibody

Anti-Integrin alpha 5 beta 1 [SG/19]

7

This antibody binds human integrin alpha 5 beta 1 near the N-terminus. Epitope mapping of SG/19 revealed that Thr82 in the 1 subunit, located at the outer face of the boundary between the I-like and hybrid domains, was the key binding determinant for this

This antibody was capable of recognizing integrin alpha 5 beta 1 expressed on the surface of K562 cells via immunofluorescence staining. This antibody also precipitated molecules with weight of 110 and 140 kDa under nonreducing conditions from K562 cells and Ramos cells, and 130 kDa when reduced. These results were similar to the ones reported for integrin alpha 5 beta 1. The effect of this antibody on migration beneath stromal cells was investigated by inoculating Nalm-6 and BMS2 cells together with this antibody. It was seen that the presence of this antibody blocked the migration of Nalm-6 cells beneath stromal cells (PMID: 1400595).

Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation

IF; IP; FC; functional assay

Research Use Only

Monoclonal

SG/19

ITGB1

integrin subunit beta 1

CD29; FNRB; glycoprotein IIa; GPIIA; integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); MDF2; MSK12; very late activation protein, beta polypeptide; VLAB; VLA-BETA

Mouse

IgG1

Integrin beta-1

Human

Human

The original antibody was generated by immunizing BALB/c mice with Nalm-6 cells.

-20°C,2°C to 8°C

12352203