Anti-Integrin alpha-L [D2-57], Rabbit IgG, kappa

Absolute Antibody

Anti-Integrin alpha-L [D2-57], Rabbit IgG, kappa

9

This antibody is specific for the LFA-1 I domain in a HA conformation.

The original antibody was used for an ELISA on LFA-1 HA I domain. This antibody was used for immunofluorescence on K562 cells with different LFA-1 conformations. Furthermore this antibody was used for flow cytometry on K562 cells with different LFA-1 conformations (Huang et al., 2006; PMID:16888085), and on IL-15-cultured T lymphocytes (Shimaoka et al., 2006; PMID:16963559). This antibody was used for an ICAM-1 blocking assay on HA cells, to determine the effect of the antibody to inhibit ligand binding. The antibody was used for a PHA-induced lymphocyte proliferation assay on PBMCs, to determine the effect of the antibody on these cells. It did indeed block PHA-induced lymphocyte proliferation (Huang et al., 2006; PMID:16888085). This antibody was used for surface plasmon resonance on LFA-1 I domain to determine its binding affinity (Shimaoka et al., 2006; PMID:16963559). The structure of this antibody was determined using x-ray crystallography (Zhang et al., 2009; PMID:19805116).

Flow Cytometry, ImmunoFluorescence, ELISA, Other Application

Research Use Only

Monoclonal

D2-57

ITGAL

integrin subunit alpha L

antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; CD11 antigen-like family member A; CD11A; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; lymphocyte function-associated antigen 1, alpha polypeptide

Rabbit

IgG

Integrin alpha-L

Human

-20°C,2°C to 8°C

12352203