Anti-Integrin alpha.sub.v.beta.sub.3 [LM609]

Absolute Antibody

Anti-Integrin alpha.sub.v.beta.sub.3 [LM609]

7

The antibody specifically recognizes alphaVbeta3 integrin. In particular, it recognizes an epitope on the alphaVbeta3 integrin headpiece apex at the interface between the beta-propeller and betaI domains, sterically hindering access of large ligands to th

The antibody inhibited the attachment of human endothelial cells to fibrinogen, von Willebrand factor, and vitronectin (Cheresh et al, 1987; pmid:2442758). The antibody was used in FACS analysis on M21 cells. The antibody was used for immunoprecipitation analysis on M21 cells. The antibody is capable of preventing M21 cell attachment to vitronectin, von Willebrand factor, fibrinogen, and the Arg-Gly-Asp peptide (Cheresh et al, 1987; pmid:2447074). Flow cytometry analysis of integrin alphanubeta3 expression profiles of HUVEC was performed using this antibody. The antibody was used immunofluorescence against integrin alphanubeta3 (Diaz et al, 2020; pmid:31780581). The antibody was used in immunohistochemistry (Mena et al, 2014; pmid:24973039). The antibody blocked the binding of fibronectin and vitronectin proteins to alphanubeta3 in a solid-phase microtiter assay. In vitro studies demonstrated the antibody inhibits fibronectin binding to alphaVbeta3 (Charo et al., 1990; pmid:1703545). The structure of the Fab fragment was determined using X-ray crystallography and the affinity between the antibody and the ectodomain of human alphanubeta3 integrin measured by surface plasmon resonance (Kd= 2.3*10^-8 M) (Borst et al., 2017; pmid:29033288). A fully humanized variant of the antibody MED-523 (Vitaxin) entered Phase I clinical trials for the treatment of cancers, resulting in no significant toxicity at any dose level (Gutheil et al., 2000; pmid:10955784). The second generation Etaracizumab (Abegrin) antibody, demonstrated increased binding affinity to alphaVbeta3 with a significant reduction in brain tumor size following alphaVbeta3-targeted radio-immunotherapy (Veeravagu et al., 2008; pmid:19010848).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

IP; IF; FC; IHC; therapheutic; crystallography; inhibition

Research Use Only

Monoclonal

LM609

ITGAV

integrin subunit alpha V

antigen identified by monoclonal antibody L230; CD51; integrin alpha-V; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; vitronectin receptor subunit alpha; VNRA; VTNR

Rabbit

IgG

Integrin alpha-V

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunizing BALB/c mice with purified cell adhesion receptor.

-20°C,2°C to 8°C

12352203