Anti-Integrin beta-3 [Y2/51]

Absolute Antibody

Anti-Integrin beta-3 [Y2/51]

7

This antibody recognises integrin beta-3 which is a receptor for multiple ligands, such as laminin, fibronectin, prothrombin and others. It is involved in platelet-platelet interactions leading to a blood clot creation. Mutations in this protein are known

This antibody has been extensively used as a marker of megakaryocytes. It is very effective in the immunohistochemical detection of platelets and megakaryocytes in formalin-fixed tissue samples as well as via Western blot and was also recommended for the identification of blood clots in blood vessels (Gatter et al., 1988). Y2/51 antibody has been also found useful in characterisation of other cells and tissues. For instance, one group used it (in concert with other antibodies) to compare immunohistochemically fibroblasts from normal and ruptured human anterior cruciate ligaments for tissue engineering purposes (Brune et al., 2007). Finally, another group performed immunofluorescence staining of HCT15 cells with this antibody and noted increased Integrin beta-3 expression in butyrate-preselected cells in a study on the acquisition of butyrate-resistance by colon cancer cells (Serpa et al., 2010).

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry

IHC; IF; FACS; WB

Research Use Only

Monoclonal

Y2/51

ITGB3

integrin subunit beta 3

antigen CD61; BDPLT16; BDPLT2; BDPLT24; CD61; GP3A; GPIIIa; GT; GT2; integrin beta 3; integrin beta chain, beta 3; integrin beta-3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); platelet membrane glycoprotein IIIa

Human

IgG1

Integrin beta-3

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

This antibody was raised by immunising Balb/c mice with PHA-stimulated peripheral blood cells and subsequent fusing spleen cells and NSl myeloma cells.

-20°C,2°C to 8°C

12352203