Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (RP1016). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant rat IL10 protein 10 ng, Lane 2: recombinant rat IL10 protein 5 ng, Lane 3: recombinant rat IL10 protein 1 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # RP1016) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa.
Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (RP1016). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant rat IL10 protein 10 ng, Lane 2: recombinant rat IL10 protein 5 ng, Lane 3: recombinant rat IL10 protein 1 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # RP1016) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa.
Anti-Interleukin-10 IL10 Antibody Picoband(r)
RP1016-DYLIGHT594
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetIl10
Overview
- SupplierBoster Bio
- Product NameAnti-Interleukin-10 IL10 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteBy Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID25325
- Target nameIl10
- Target descriptioninterleukin 10
- Target synonymsCSIF; cytokine synthesis inhibitory factor; If2a; IL-10; IL10X; interferon 2a; interleukin-10
- HostRabbit
- IsotypeIgG
- Protein IDP29456
- Protein NameInterleukin-10
- Scientific DescriptionBoster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1016. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityRat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203