
Figure 1. Western blot analysis of ITPA using anti-ITPA antibody (A02304). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPA antigen affinity purified polyclonal antibody (Catalog # A02304) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPA at approximately 21 kDa. The expected band size for ITPA is at 21 kDa.
Anti-ITPA Antibody Picoband(r)
A02304-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetITPA
Overview
- SupplierBoster Bio
- Product NameAnti-ITPA Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateRPE
- Gene ID3704
- Target nameITPA
- Target descriptioninosine triphosphatase
- Target synonymsC20orf37; DEE35; dJ794I6.3; epididymis secretory sperm binding protein; HLC14-06-P; inosine triphosphatase (nucleoside triphosphate pyrophosphatase); inosine triphosphate pyrophosphatase; inosine triphosphate pyrophosphohydrolase; ITPase; My049; non-canonical purine NTP pyrophosphatase; non-standard purine NTP pyrophosphatase; NTPase; nucleoside-triphosphate diphosphatase; putative oncogene protein HLC14-06-P
- HostRabbit
- IsotypeIgG
- Protein IDQ9BY32
- Protein NameInosine triphosphate pyrophosphatase
- Scientific DescriptionBoster Bio Anti-ITPA Antibody Picoband® catalog # A02304. Tested in ELISA, IF, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203