More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-JLP/SPAG9 Antibody Picoband(r)
Figure 1. Western blot analysis of JLP/SPAG9 using anti-JLP/SPAG9 antibody (A02751-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JLP/SPAG9 antigen affinity purified polyclonal antibody (Catalog # A02751-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JLP/SPAG9 at approximately 190 kDa. The expected band size for JLP/SPAG9 is at 190 kDa.
Figure 1. Western blot analysis of JLP/SPAG9 using anti-JLP/SPAG9 antibody (A02751-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JLP/SPAG9 antigen affinity purified polyclonal antibody (Catalog # A02751-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JLP/SPAG9 at approximately 190 kDa. The expected band size for JLP/SPAG9 is at 190 kDa.
Figure 1. Western blot analysis of JLP/SPAG9 using anti-JLP/SPAG9 antibody (A02751-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JLP/SPAG9 antigen affinity purified polyclonal antibody (Catalog # A02751-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JLP/SPAG9 at approximately 190 kDa. The expected band size for JLP/SPAG9 is at 190 kDa.

Anti-JLP/SPAG9 Antibody Picoband(r)

A02751-1-10UG
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSPAG9
10 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-JLP/SPAG9 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID9043
  • Target name
    SPAG9
  • Target description
    sperm associated antigen 9
  • Target synonyms
    cancer/testis antigen 89; c-Jun NH2-terminal kinase-associated leucine zipper protein; C-Jun-amino-terminal kinase-interacting protein 4; CT89; HLC4; HLC6; HLC-6; human lung cancer oncogene 6 protein; JIP4; JIP-4; JLP; JNK interacting protein; JNK/SAPK-associated protein; lung cancer oncogene 4; Max-binding protein; mitogen-activated protein kinase 8-interacting protein 4; PHET; PIG6; proliferation-inducing gene 6; protein highly expressed in testis; sperm associated antigen 9 variant 5; sperm surface protein; sunday driver 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60271
  • Protein Name
    C-Jun-amino-terminal kinase-interacting protein 4
  • Scientific Description
    Boster Bio Anti-JLP/SPAG9 Antibody Picoband® catalog # A02751-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203