Anti-Junin virus GP1 [GD01], Mouse IgG2a, Fc Silent(tm), kappa

Absolute Antibody

Anti-Junin virus GP1 [GD01], Mouse IgG2a, Fc Silent(tm), kappa

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This antibody is specific for the GP1 subunit of the JUNV surface envelope glycoprotein GPC.

The original version of this antibody (mouse IgG2a) successfully bound Junin virus (XJ45 strain) GP1 in an ELISA. This antibody was used for the indirect immunofluorescent staining of Junin virus-infected Vero cells. This antibody reacted with Junin GP1 in immunoprecipitation and western blot. This antibody successfully neutralized the Junin virus in an in vitro assay (Sanchez et al., 1989; PMID: 2471803). The GP1-bound Fab version of this antibody was crystallized, and its structure was determined. This antibody neutralized the active form of JUNV by disrupting the interaction between GP1 and TfR1. A competition assay showed that IgGs from survivors of AHF did not compete with this antibody (Mahmutovic et al., 2015; PMID: 26651946). This antibody exhibited potent neutralization of the Candid #1 JUNV strain in a plaque neutralization assay (Zeitlin et al., 2016; PMID: 27044104).

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

GD01

Mouse

IgG2a

Pre-glycoprotein polyprotein GP complex

This is a reformatted mouse IgG2a Fc Silent™ antibody, based on the original mouse IgG2a format, created for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203