Anti-Kell [MIMA-8]

Absolute Antibody

Anti-Kell [MIMA-8]

7

MIMA-8 was shown to be specific for the Jsb epitope of the Kell protein using flow cytometry, whereby the mAb agglutinated Jsb+ , but not Jsa+Jsb-, erythrocytes (Chu et al, 1999). These erythrocytes also lacked other high-incidence Kell protein antigens.

MIMA-8 was shown, by flow cytometry, to react to the Jsb epitope of Kell protein (Chu et al, 1999). As a result, MIMA-8 could be used as a diagnostic reagent for cell typing of patient blood samples, or as a research tool. Notably, the Jsb epitope is known to be located close to the Kell enzymatic active site, and thus MIMA-8 could be used to study the structure-function relationship at this region.

Flow Cytometry

FC

Research Use Only

Monoclonal

MIMA-8

Kel

Kell blood group

CD238; Ece3; Kel blood group protein; kell blood group glycoprotein homolog; Kell blood group, metallo-endopeptidase; Kell protein

Human

IgG4

Kell blood group glycoprotein homolog

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

Murine antibodies were produced by immunising BALB/c mice with the mouse erythroleukaemic (MEL) cell line stably expressing high levels of wildtype, full-length human Kell protein. This overexpression allowed several epitopes of Kell protein to serve as the immunogen, resulting in identification of several anti-Kell mAbs with different specificities, of which MIMA-8 targeted the Jsb antigen. Splenocytes were obtained from immunised mice and fused with the P3X63Ag8.653 myeloma cell line to produce a hybridoma.

-20°C,2°C to 8°C

12352203