Figure 1. Western blot analysis of Keratocan using anti-Keratocan antibody (PB9653). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Testis Whole Cell Lysate at 40ug, Lane 2: Mouse Skeletal Muscle Whole Cell Lysate at 40ug, Lane 3: MCF-7 Whole Cell Lysate at 40ug, Lane 4: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keratocan antigen affinity purified polyclonal antibody (Catalog # PB9653) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keratocan at approximately 50 kDa. The expected band size for Keratocan is at 40 kDa.
Figure 1. Western blot analysis of Keratocan using anti-Keratocan antibody (PB9653). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Testis Whole Cell Lysate at 40ug, Lane 2: Mouse Skeletal Muscle Whole Cell Lysate at 40ug, Lane 3: MCF-7 Whole Cell Lysate at 40ug, Lane 4: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keratocan antigen affinity purified polyclonal antibody (Catalog # PB9653) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keratocan at approximately 50 kDa. The expected band size for Keratocan is at 40 kDa.
Anti-Keratocan Antibody Picoband(r)
PB9653-HRP
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human, Mouse
TargetKERA
Overview
- SupplierBoster Bio
- Product NameAnti-Keratocan Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID11081
- Target nameKERA
- Target descriptionkeratocan
- Target synonymsCNA2; keratan sulfate proteoglycan keratocan; keratocan; KTN; SLRR2B
- HostRabbit
- IsotypeIgG
- Protein IDO60938
- Protein NameKeratocan
- Scientific DescriptionBoster Bio Anti-Keratocan Antibody Picoband® catalog # PB9653. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203