Immunohistochemical analysis of paraffin-embedded human tonsil, using Ki67 Antibody (M00254) MKI67 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MKI67 Antibody (M00254)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human tonsil, using Ki67 Antibody (M00254) MKI67 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MKI67 Antibody (M00254)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Ki67 MKI67 Rabbit Monoclonal Antibody
M00254
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMKI67
Overview
- SupplierBoster Bio
- Product NameAnti-Ki67 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDEAH-13
- FormulationLiquid
- Gene ID4288
- Target nameMKI67
- Target descriptionmarker of proliferation Ki-67
- Target synonymsantigen identified by monoclonal antibody Ki-67; antigen Ki67; KIA; MIB-; MIB-1; Molecular Immunology Borstel antibody 1; PPP1R105; proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105
- HostRabbit
- IsotypeIgG
- Protein IDP46013
- Protein NameProliferation marker protein Ki-67
- Scientific DescriptionBoster Bio Anti-Ki67 MKI67 Rabbit Monoclonal Antibody catalog # M00254. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203
References
- TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer. Zhou C et al., 2021 Oct, AutophagyRead more
- LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/beta-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes. Zhou C et al., 2020 Jul 29, Mol CancerRead more