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ProductsBack/Anti-DNA/RNA-binding protein KIN17 KIN Antibody Picoband(r)
Anti-KIN antibody, PA2238, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: Human Placenta Tissue Lysate Lane 3: Rat Testis Tissue Lysate
Anti-KIN antibody, PA2238, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: Human Placenta Tissue Lysate Lane 3: Rat Testis Tissue Lysate
Anti-KIN antibody, PA2238, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: Human Placenta Tissue Lysate Lane 3: Rat Testis Tissue Lysate

Anti-DNA/RNA-binding protein KIN17 KIN Antibody Picoband(r)

PA2238
Boster Bio
ApplicationsWestern Blot
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetKIN
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-KIN Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID22944
  • Target name
    KIN
  • Target description
    Kin17 DNA and RNA binding protein
  • Target synonyms
    binding to curved DNA; BTCD; DNA/RNA-binding protein KIN17; KIN, antigenic determinant of recA protein homolog; KIN17; Rts2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60870
  • Protein Name
    DNA/RNA-binding protein KIN17
  • Scientific Description
    Boster Bio Anti-DNA/RNA-binding protein KIN17 KIN Antibody catalog # PA2238. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity Supplier
    Human, Mouse, Rat, Chicken
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KIN using anti-KIN antibody (PB9607). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIN antigen affinity purified polyclonal antibody (Catalog # PB9607) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIN at approximately 45 kDa. The expected band size for KIN is at 45 kDa.
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