Anti-Kinetochore protein NDC80 homolog [Hec1 (9G3)]

Absolute Antibody

Anti-Kinetochore protein NDC80 homolog [Hec1 (9G3)]

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This antibody is widely used in studies involving the role of Hec1 protein in mitosis. This antibody can immunoprecipitate human Hec1 protein from nuclear, cytoplasmic, membrane and other components of T24 human bladder carcinoma cells. This antibody was capable of recognizing a 76-KDa protein in western blot. It was reported that when T24 human bladder carcinoma cells were synchronized in S phase and microinjected with this antibody the cells contained multiple, fragmented nuclei (PMID: 9315664). Preincubation of the antibody with C-2 peptide (comprising amino acids 200-215 of Hec1) prevented the antibody from recognizing kinetochores in immunofluorescence assays. Immunofluorescent staining of HeLa and PtK1 cells in metaphase with 9G3 and another anti-Spc24 antibody revealed that the N terminus of Hec1 was always exterior to Spc24 at kinetochores of bi-oriented chromosomes. It was further reported that PtK1 cells injected with this antibody exhibited mitotic defects (PMID: 17129782). A recombinantly generated version of this antibody rMAb-Hec1 robustly recognized kinetochores however, the original antibody exhibited more spindle staining and overall background staining when compared to the recombinant version (PMID: 34970967).

ImmunoFluorescence, ImmunoPrecipitation, Western Blot

IP; WB; IF

Research Use Only

Kinetochore protein NDC80 homolog

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203