IHC analysis of Kallikrein 1 using anti-Kallikrein 1 antibody (PA1633). Kallikrein 1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Kallikrein 1 Antibody (PA1633) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Kallikrein 1 using anti-Kallikrein 1 antibody (PA1633). Kallikrein 1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Kallikrein 1 Antibody (PA1633) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Kallikrein 1/Ngfg Antibody Picoband(r)
PA1633
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetKlk1b3
Overview
- SupplierBoster Bio
- Product NameAnti-KLK1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID24594
- Target nameKlk1b3
- Target descriptionkallikrein 1-related peptidase B3
- Target synonymsgamma-NGF; kallikrein 1; kallikrein 1-related peptidase B3; kallikrein a4; Klk1; Klk1c1; Klna4; nerve growth factor gamma chain; nerve growth factor gamma polypeptide; Ngfg; pancreatic kallikrein; PS kallikrein; rGK-1; rK-1; true tissue kallikrein
- HostRabbit
- IsotypeIgG
- Protein IDP00758
- Protein NameKallikrein-1
- Scientific DescriptionBoster Bio Anti-Kallikrein 1/Ngfg Antibody catalog # PA1633. Tested in IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityRat
- Reactivity SupplierRat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203