Anti-LAG-3 [C9B7W]

Absolute Antibody

Anti-Lag3 [C9B7W]

7

This antibody is specific for an epitope in the D2 domain of murine LAG-3.

This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild-type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen-induced IL-2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).

Flow Cytometry, ImmunoPrecipitation, Neutralisation/Blocking

FC; IP; blocking

Research Use Only

Monoclonal

C9B7W

Lag3

lymphocyte-activation gene 3

activation-induced cytidine deaminase-linked autoimmunity protein; aida; CD223; LAG; LAG-3; Ly6; Ly66; lymphocyte activation gene 3 protein

Mouse

IgG2a

Lymphocyte activation gene 3 protein

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.

-20°C,2°C to 8°C

12352203