Anti-LAM [CS-35]

Absolute Antibody

Anti-LAM [CS-35]

9

This antibody binds lipoarabinomannan and recognizes the common terminal branched saraf-containing arrangement.

This antibody has been used as a reference antibody for the characterization of mAbs against LAM and to study a variety of cellular functions, such as the role of LAM in the interaction of mycobacteria with macrophages. This antibody can recognize LAM from Mycobacterium tuberculosis strain Erdman and H37Ra and Mycobacterium bovis in a western blot assay (Chatterjee et al., 1992; PMID: 1556132) (Prinzis et al., 1993; PMID: 8277248). This antibody was also used for the study of the interaction of LAMs with lymphocytes and monocytes using immunofluorescence assays and flow cytometry (Ilangumaran et al., 1995; PMID: 7636199). Epitope mapping of this antibody was done using ELISA (Kaur et al., 2002; PMID: 12368438). The binding of this antibody to LAM has been thoroughly characterized (Rademacher et al., 2007; PMID: 17672460).

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA

Research Use Only

Monoclonal

CS-35

Mouse

IgG2b

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Bacteria

-20°C,2°C to 8°C

12352203