Western blot analysis of LBP using anti-LBP antibody (A00809-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human LBP protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBP antigen affinity purified polyclonal antibody (Catalog # A00809-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBP at approximately 65KD. The expected band size for LBP is at 51KD.
Western blot analysis of LBP using anti-LBP antibody (A00809-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human LBP protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBP antigen affinity purified polyclonal antibody (Catalog # A00809-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBP at approximately 65KD. The expected band size for LBP is at 51KD.
Anti-LBP Antibody Picoband(r)
A00809-2
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetLBP
Overview
- SupplierBoster Bio
- Product NameAnti-LBP Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID3929
- Target nameLBP
- Target descriptionlipopolysaccharide binding protein
- Target synonymsBPI fold containing family D, member 2; BPIFD2; lipopolysaccharide-binding protein; LPS-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP18428
- Protein NameLipopolysaccharide-binding protein
- Scientific DescriptionBoster Bio Anti-LBP Antibody Picoband® catalog # A00809-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203