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IHC analysis of Leptin using anti-Leptin antibody (A00479-4). Leptin was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Leptin using anti-Leptin antibody (A00479-4). Leptin was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Leptin using anti-Leptin antibody (A00479-4). Leptin was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-Leptin Antibody Picoband(r)

Research Use Only
A00479-4
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityMouse, Rat
TargetLep
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Leptin Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Formulation
    Lyophilized
  • Gene ID16846
  • Target name
    Lep
  • Target description
    leptin
  • Target synonyms
    leptin; ob; obese; obesity factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41160
  • Protein Name
    Leptin
  • Scientific Description
    Boster Bio Anti-Leptin Antibody Picoband® catalog # A00479-4. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid. Chen L et al., 2017 Mar, Endocrine
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