Figure 1. Western blot analysis of LPXN using anti-LPXN antibody (PA2195). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPXN antigen affinity purified polyclonal antibody (Catalog # PA2195) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPXN at approximately 43 kDa. The expected band size for LPXN is at 43 kDa.
Figure 1. Western blot analysis of LPXN using anti-LPXN antibody (PA2195). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPXN antigen affinity purified polyclonal antibody (Catalog # PA2195) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPXN at approximately 43 kDa. The expected band size for LPXN is at 43 kDa.
Anti-Leupaxin/LPXN Antibody Picoband(r)
PA2195-PE
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHamster, Human, Mouse
TargetLPXN
Overview
- SupplierBoster Bio
- Product NameAnti-Leupaxin/LPXN Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateRPE
- Gene ID9404
- Target nameLPXN
- Target descriptionleupaxin
- Target synonymsLDPL; leupaxin
- HostRabbit
- IsotypeIgG
- Protein IDO60711
- Protein NameLeupaxin
- Scientific DescriptionBoster Bio Anti-Leupaxin/LPXN Antibody catalog # PA2195. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203