Anti-LeX [291-2G3-A]

Absolute Antibody

Anti-LeX [291-2G3-A]

9

The antibody binds to Lewis X trisaccharide, a carbohydrade pivotal in mediating specific cell-cell interactions, with an affinity of 11 microM.

The antibody binds specifically to Lewis X, a tetrasaccharide carbohydrate blood-group antigen involved in cell-to-cell adhesion. The antigen is involved in human fertilisation, as it is present on the terminus of N- and O-glycans of the zona pellucida of egg cells, which are recognised by sperm cells This interaction allows fusion and fertilisation to occur. LeX also plays a role in leukocyte tethering as they travel through the bloodstream as it can interact with L-selectin, P-selectin and E-selectin. LeX is also present on neutrophils, mediating phagocytosis and chemotaxis, and is expressed in patients with Hodgkin disease, B-cell chronic lymphocytic leukemia, acute lymphoblastic leukemias and acute nonlymphocytic leukemia. It is also expressed as SSEA-1 on mouse embryonic stem cells. LeX also plays a role in human resistance to the parasitic disease schistosomiasis.

ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

291-2G3-A

Human

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203