Anti-LHRH [SMI41]

Absolute Antibody

Anti-LHRH [SMI41]

7

SMI41 reacts with LHRH with an affinity of 10 pM and is specific for its C-terminal pentapeptide (G-L-R-P-G).

SMI-41 has been used in immunocytochemistry experiments to detect the expression of mature LHRH in the brain detect cells containing mature LHRH peptide (Avigdor 2004). SMI-41 also has been expressed in mice via injection of a replication-incompetent, recombinant adeno-associated virus designed to express the antibody. This has been used to cofer long-term infertility in male and female mice (Li 2015).

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, RadioImmunoAssay

IHC; IHC-P; RIA; therapeutic

Research Use Only

Monoclonal

SMI41

GNRHR

gonadotropin releasing hormone receptor

gnRH receptor; gnRH-R; GNRHR1; gonadotropin-releasing hormone (type 1) receptor 1; gonadotropin-releasing hormone receptor; GRHR; HH7; leutinizing hormone releasing horomone receptor; leutinizing-releasing hormone receptor; LHRHR; LRHR; luliberin receptor; type I GnRH receptor

Porcine

IgG1

Gonadotropin-releasing hormone receptor

This chimeric pig antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

LHRH diacetate tetrahydrate was conjugated to potato acid phosphatase and an emulsion composed of the conjugate and Freunds complete adjuvant was injected into the hind limb of female CAF1 mice. Immune spleen cells were fused with P3-X63-Ag8.653/3 myeloma cells by a modification of the original cell fusion method using polyethylene glycol. A hybridoma screening assays were used to select for high affinity monoclonal antibodies.

-20°C,2°C to 8°C

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