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Figure 1. Western blot analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 antigen affinity purified polyclonal antibody (PB9608) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin-2/NGAL/LCN2 at approximately 22 kDa. The expected band size for Lipocalin-2/NGAL/LCN2 is at 22 kDa.
Figure 1. Western blot analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 antigen affinity purified polyclonal antibody (PB9608) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin-2/NGAL/LCN2 at approximately 22 kDa. The expected band size for Lipocalin-2/NGAL/LCN2 is at 22 kDa.
Figure 1. Western blot analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 antigen affinity purified polyclonal antibody (PB9608) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin-2/NGAL/LCN2 at approximately 22 kDa. The expected band size for Lipocalin-2/NGAL/LCN2 is at 22 kDa.

Anti-Lipocalin 2/LCN2 Antibody Picoband(r)

Research Use Only
PB9608
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityMouse, Rat
TargetLcn2
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Lipocalin-2/NGAL Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Formulation
    Lyophilized
  • Gene ID16819
  • Target name
    Lcn2
  • Target description
    lipocalin 2
  • Target synonyms
    24p; 24p3; AW212229; N; neu-related lipocalin; neutrophil gelatinase-associated lipocalin; NRL; oncogene 24p3; p25; secreted inducible protein 24; siderocalin LCN2; Sip24; SV-40-induced 24P3 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11672
  • Protein Name
    Neutrophil gelatinase-associated lipocalin
  • Scientific Description
    Boster Bio Anti-Lipocalin 2/LCN2 Antibody Picoband® catalog # PB9608. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Dexmedetomidine alleviates lipopolysaccharide-induced acute kidney injury by inhibiting the NLRP3 inflammasome activation via regulating the TLR4/NOX4/NF-kappaB pathway. Yao Y et al., 2019 Oct, J Cell Biochem
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