Anti-Lipoprotein lipase [5D2]

Absolute Antibody

Anti-Lipoprotein lipase [5D2]

9

The antibody is specific for lipoprotein lipase (LPL) and it binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The antibody does not cross react with mouse LPL. LPL is a key enzyme in triglyceride metabolism, it catalyzes the hydrolys

LPL was immunoprecipitated from postheparin plasma using this antibody. The antibody was used both to capture LPL and to detect the bound LPL in a sandwich ELISA, indicating the epitope to be present in duplicate. In another sandwich ELISA analysis, the antibody was used to detect the LPL antigen (Peterson et al., 1992, PMID: 1279089). The antibody detected LPL from human, chicken and guinea pig by western blot analysis. The specificity of the original format of the antibody was confirmed by ELISA analysis (Chang et al., 1998; PMID: 9831623). Pretreatment of LPL with the antibody totally suppressed LPL-induced monocyte adhesion to endothelial cells (Mamputu et al., 1997; PMID: 9323582). Further, LDL was immunoneutralized with the antibody, totally suppressing LPL-induced VSMC proliferation (Mamputu et al., 2000; PMID: 11031206). Immunofluorescence was performed on LPL expressed on CHO-K1 cells using this antibody (Voss et al., 2011; PMID: 21518912). The binding affinity of the antibody to a synthetic LPL peptide containing the Trp-rich loop of human, mouse, bovine, rat and chicken, was measured by surface plasmon resonance.The binding affinity of the antibody to the human, bovine and chicken LPL peptides was high (KD of 0.19, 0.78 nM and 0.34 nM, respectively), the binding affinity for the rat LPL peptide was reduced (Kd= 2.99nM), and no binding to the Trp-rich peptide from mouse LPL was detected. The antibody detected LPL by western blot analysis. By immunocytochemistry, the antibody bound avidly to the wildtype human LPL on CHO cells. The crystal structure of the Fab version of the antibody was solved (Luz et al., 2020; PMID: 32690595).

ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

5D2

LPL

lipoprotein lipase

HDLCQ11; LIPD; lipoprotein lipase; phospholipase A1

Rabbit

IgG

Lipoprotein lipase

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Bovine, Chicken, Feline, Guinea Pig, Human, Rat

-20°C,2°C to 8°C

12352203