Anti-Ly6G [1A8]

Absolute Antibody

Anti-Ly6G [1A8]

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Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.

Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022; PMID: 36359801).

Flow Cytometry, Other Application

FC; depletion

Research Use Only

Monoclonal

1A8

Ly6g

lymphocyte antigen 6 complex, locus G

Gr; Gr-; Gr1; Gr-1; Ly-6; Ly-6G; ly-6G.1; lymphocyte antigen 6G

Rat

IgG2a

Lymphocyte antigen 6G

This antibody was created using our proprietary Fc Silent™ engineered Fc domain, matching the original species and isotype, but containing key point mutations that abrogate binding of Fc Receptor and complement.

Mouse

Mouse

LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells

-20°C,2°C to 8°C

12352203