Anti-M-CSF [Caline], Human IgG1, lambda

Absolute Antibody

Anti-M-CSF [Caline], Human IgG1, lambda

9

This antibody is specific for human M-CSF. It has been shown to cross-react with cynomolgus monkey M-CSF. M-CSF is a secreted cytokine that influences hematopoietic stem cells to differentiate into macrophages or other related cell types.

The original version of this antibody (human IgG1) was tested for its specificity for human M-CSF and cross-reactivity for cynomolgus M-CSF using ELISA. KD values of 14 pM and 16 pM were measured. This antibody binds M-CSF expressed on cells, as shown in a FACS assay that exhibited an EC50 value of 9.8 nM and 14 nM against M-CSF-expressing transfected CHO cells and MDA-MB 231 cells, respectively. This antibody successfully inhibited human and cynomolgus M-CSF binding to recombinant human M-CSF receptor with an IC50 of 6.1 pM and 6.3 pM, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by recombinant and native human and cynomolgus M-CSF with an IC50 of 3.1 pM and 17.7 pM for recombinant and native human M-CSF, respectively, and 4.3 pM and 106.9 pM for recombinant and native cynomolgus M-CSF, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by membrane-bound human M-CSF (expressed by stably transfected CHO cells) (US10081675B2).

Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

Caline

CSF1

colony stimulating factor 1

colony stimulating factor 1 (macrophage); CSF-1; lanimostim; macrophage colony stimulating factor 1; macrophage colony-stimulating factor 1; MCSF

Human

IgG1

Macrophage colony-stimulating factor 1

Human, Monkey

-20°C,2°C to 8°C

12352203