Anti-MAGEA3 [21B4]

Absolute Antibody

Anti-MAGEA3 [21B4]

7

This antibody binds human MAGEA3 and MAGEA6, but does not cross react with other members of the MAGE family like MAGE-A1, MAGE-A4 and MAGE-A10. MAGE-A3 is a tumor specific antigen and it is expressed on many tumors like melanoma, non-small cell lung cance

The binding specificity of this antibody towards MAGE-A3 was determined using ELISA. The original antibody cross reacts with MAGEA6 but does not react with other members of the MAGE family in an ELISA or western blot assay. This antibody was used in the detection of HEK293T cells expressing MAGEA3 and MAGEA6 using flow cytometry. The original IgG1 antibody was capable of immunoprecipitating MAGE-A3 from lysates of SK-MEL-37 cells endogenously expressing MAGEA3. The original antibody was also used in the immunohistochemical staining of tissues form normal testis and squamous cell carcinoma of the lung (US20140093514).

Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry

ELISA; FC; IP; WB; IHC

Research Use Only

Monoclonal

21B4

MAGEA3

MAGE family member A3

antigen MZ2-D; cancer/testis antigen 1.3; cancer/testis antigen family 1, member 3; CT1.3; HIP8; HYPD; MAGE3; MAGE-3 antigen; MAGEA6; melanoma antigen family A, 3; melanoma antigen family A3; melanoma-associated antigen 3

Mouse

IgG2b

Melanoma-associated antigen 3

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was isolated from a patient suffering from a tumor expressing a tumor specific antigen like MAGE-A3.

-20°C,2°C to 8°C

12352203