Figure 1. Western blot analysis of MBNL3 using anti-MBNL3 antibody (A08298). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBNL3 antigen affinity purified polyclonal antibody (Catalog # A08298) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBNL3 at approximately 27 kDa. The expected band size for MBNL3 is at 39 kDa.
Figure 1. Western blot analysis of MBNL3 using anti-MBNL3 antibody (A08298). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBNL3 antigen affinity purified polyclonal antibody (Catalog # A08298) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBNL3 at approximately 27 kDa. The expected band size for MBNL3 is at 39 kDa.
Anti-MBNL3 Antibody Picoband(r)
A08298-HRP
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetMBNL3
Overview
- SupplierBoster Bio
- Product NameAnti-MBNL3 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID55796
- Target nameMBNL3
- Target descriptionmuscleblind like splicing regulator 3
- Target synonymsCHCR; Cys3His CCG1-required protein; MBLX; MBLX39; MBXL; muscleblind-like 3; muscleblind-like protein 3; muscleblind-like X-linked protein
- HostRabbit
- Protein IDQ9NUK0
- Protein NameMuscleblind-like protein 3
- Scientific DescriptionBoster Bio Anti-MBNL3 Antibody Picoband® catalog # A08298. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203